Maternal HLA-G*01:01:01:04 protects from anti-HLA-class II immunization in pregnant women

Hum Immunol. 2019 Feb;80(2):120-125. doi: 10.1016/j.humimm.2018.11.003. Epub 2018 Nov 7.


Factors determining anti-HLA immunization are poorly understood, although anti-HLA immunization following pregnancy is well described. The HLA-G molecule has been extensively described for its implication in immunological tolerance, especially during pregnancy. Transplant studies show an association between HLA-G haplotypes and alloimmunization. Our aim was to investigate the association of HLA-G haplotypes with anti-HLA class I and II immunization in a cohort of women having experienced one or more pregnancies and with no transfusion history. Maternal blood samples (n=270) collected at delivery and formerly screened for anti-HLA antibodies, HLA-A and HLA-B antigens, were screened by NGS for HLA-G gene polymorphism. Univariate analysis further confirmed that the number of pregnancies was significantly associated with anti-HLA class I immunization, whereas no other variable remained significant after Bonferroni correction. Our results showed however that anti-HLA class II immunization was associated with the number of children whereas the HLA-G*01:01:01:04 allele was protective against this immunization.

Keywords: HLA-G; anti-HLA imunization; haplotypes; pregnancy; regulatory regions.

MeSH terms

  • Adolescent
  • Adult
  • Cohort Studies
  • Female
  • Genetic Association Studies
  • Gravidity
  • HLA-G Antigens / genetics*
  • Histocompatibility Antigens Class II / immunology*
  • Humans
  • Immune Tolerance
  • Immunization
  • Polymorphism, Genetic
  • Pregnancy
  • Young Adult


  • HLA-G Antigens
  • HLA-G*01:01:01 antigen
  • Histocompatibility Antigens Class II