Developing Transgenic Agronomic Traits for Crops: Targets, Methods, and Challenges

Methods Mol Biol. 2019:1864:343-365. doi: 10.1007/978-1-4939-8778-8_22.

Abstract

The last two decades have witnessed a surge of investment by the agricultural biotechnology industry in the development of transgenic agronomic traits. These are traits that improve yield performance by modifying endogenous physiological processes such as energy capture, nutrient utilization, and stress tolerance. In this chapter we provide a foundation for understanding these fundamental processes and then outline approaches that have been taken to use this knowledge for yield improvement. We characterize the current status of product development pipelines in the industry and illustrate the trait discovery process with three important examples-bacterial cold-shock proteins, alanine aminotransferase, and auxin-regulated genes. The challenges with developing and commercializing an agronomic trait product are discussed.

Keywords: Agronomic traits; Nitrogen-use efficiency; Stress tolerance; Transgenic traits; Yield.

Publication types

  • Review

MeSH terms

  • Biotechnology / instrumentation
  • Biotechnology / methods*
  • Biotechnology / trends
  • Crops, Agricultural / genetics*
  • Phenotype
  • Plants, Genetically Modified / genetics*
  • Quantitative Trait Loci / genetics*