Specimen Pooling as a Diagnostic Strategy for Microbiologic Confirmation in Children with Intrathoracic Tuberculosis

Pediatr Infect Dis J. 2019 Jun;38(6):e128-e131. doi: 10.1097/INF.0000000000002240.


Three-hundred four young children with suspected pulmonary tuberculosis had a gastric aspirate, induced sputum and nasopharyngeal aspirate collected on each of 2 consecutive weekdays. Specimens collected on the second day were pooled in the laboratory for each child individually. The diagnostic yield by Xpert and culture from pooled specimens was not significantly different to a single gastric aspirate.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Child
  • Child, Preschool
  • Colony Count, Microbial
  • Female
  • Humans
  • Infant
  • Male
  • Mycobacterium tuberculosis / isolation & purification*
  • Prospective Studies
  • Sensitivity and Specificity
  • Specimen Handling / methods*
  • Sputum / microbiology*
  • Thorax / microbiology*
  • Tuberculosis, Pulmonary / diagnosis*