A web server for comparative analysis of single-cell RNA-seq data

Nat Commun. 2018 Nov 13;9(1):4768. doi: 10.1038/s41467-018-07165-2.

Abstract

Single cell RNA-Seq (scRNA-seq) studies profile thousands of cells in heterogeneous environments. Current methods for characterizing cells perform unsupervised analysis followed by assignment using a small set of known marker genes. Such approaches are limited to a few, well characterized cell types. We developed an automated pipeline to download, process, and annotate publicly available scRNA-seq datasets to enable large scale supervised characterization. We extend supervised neural networks to obtain efficient and accurate representations for scRNA-seq data. We apply our pipeline to analyze data from over 500 different studies with over 300 unique cell types and show that supervised methods outperform unsupervised methods for cell type identification. A case study highlights the usefulness of these methods for comparing cell type distributions in healthy and diseased mice. Finally, we present scQuery, a web server which uses our neural networks and fast matching methods to determine cell types, key genes, and more.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brain
  • Computational Biology / methods
  • Databases, Genetic
  • Gene Expression Regulation
  • Genetic Markers
  • Internet
  • Macrophages
  • Mice
  • Neural Networks, Computer
  • Protein Interaction Mapping
  • RNA, Small Cytoplasmic / analysis*
  • Sequence Analysis, RNA / methods*
  • Single-Cell Analysis / methods*
  • Software*

Substances

  • Genetic Markers
  • RNA, Small Cytoplasmic