Isolation of Proteins from Polyacrylamide Gels

Stefanie Koristka; Claudia Arndt; Ralf Bergmann; Michael Bachmann

doi:10.1007/978-1-4939-8793-1_38

Isolation of Proteins from Polyacrylamide Gels

Methods Mol Biol. 2019:1855:461-465. doi: 10.1007/978-1-4939-8793-1_38.

Authors

Stefanie Koristka¹, Claudia Arndt¹, Ralf Bergmann¹, Michael Bachmann²

Affiliations

¹ Institute of Radiopharmaceutical Cancer Research, Department of Radio-/Tumorimmunology, Helmholtz-Zentrum Dresden Rossendorf (HZDR), Dresden, Germany.
² Institute of Radiopharmaceutical Cancer Research, Department of Radio-/Tumorimmunology, Helmholtz-Zentrum Dresden Rossendorf (HZDR), Dresden, Germany. m.bachmann@hzdr.de.

PMID: 30426439
DOI: 10.1007/978-1-4939-8793-1_38

Abstract

Minute amounts of proteins are required for immunization of mice for the development of antibodies including monoclonal antibodies. Here we describe a rapid procedure for the isolation of proteins from polyacrylamide gels after sodium dodecyl sulfate polyacrylamide gel electrophoresis in sufficient amounts for immunization of animals.

Keywords: Isolation of proteins; Polyacrylamide gels.

MeSH terms

Acrylic Resins / chemistry*
Animals
Antigens / isolation & purification
Electrophoresis, Polyacrylamide Gel / instrumentation
Electrophoresis, Polyacrylamide Gel / methods*
Equipment Design
Mice
Powders
Proteins / isolation & purification*
Staining and Labeling / methods

Substances

Acrylic Resins
Antigens
Powders
Proteins
polyacrylamide gels