Ultrarapid Sodium Dodecyl Sulfate Polyacrylamide Mini-Gel Electrophoresis

Methods Mol Biol. 2019:1855:491-494. doi: 10.1007/978-1-4939-8793-1_42.

Abstract

We describe here an ultrafast method for electrophoresing proteins on SDS-PAGE. Previously we reported a method to complete SDS-PAGE and immunoblotting in an hour, including electrophoresing proteins at 70°C in 10 min. Here we show that we can electrophorese molecular weight standards and bovine serum albumin on a 4-20% gradient gel in well under 10 min using heated (44 °C) Laemmli running buffer and high voltage.

Keywords: Gradient gel; Heat; Molecular weight standards; Precast gel; SDS-PAGE.

MeSH terms

  • Animals
  • Cattle
  • Electrophoresis, Polyacrylamide Gel / economics
  • Electrophoresis, Polyacrylamide Gel / instrumentation
  • Electrophoresis, Polyacrylamide Gel / methods*
  • Heating
  • Molecular Weight
  • Proteins / analysis*
  • Proteins / isolation & purification
  • Serum Albumin, Bovine / analysis
  • Serum Albumin, Bovine / isolation & purification
  • Sodium Dodecyl Sulfate / chemistry
  • Time Factors
  • Tromethamine / chemistry

Substances

  • Laemmli buffer
  • Proteins
  • Tromethamine
  • Serum Albumin, Bovine
  • Sodium Dodecyl Sulfate