Single Locked Nucleic Acid-enhanced nanopore genetic discrimination of pathogenic serotypes and cancer driver mutations

Annu Int Conf IEEE Eng Med Biol Soc. 2018 Jul:2018:4492-4495. doi: 10.1109/EMBC.2018.8513177.

Abstract

Rapid and accurate detection of single-nucleotide polymorphism (SNP) in pathogenic mutants is crucial for broad fields from food safety monitoring to disease diagnostics and prognosis. Here, we developed a nanopore single-molecule sensor, coupled with the locked nucleic acid (LNA) technique, to accurately discriminate SNPs for detection of Shiga toxin producing Escherichia coli (STEC) O157:H7 pathogen serotype, and cancer-derived driver mutations EGFR L858R and KRAS G12D. This sensitive method, with a simplified, low cost, easy-to-operate LNA design, can be applied in food science and medical detection that need rapid and accurate determination of genetic variations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • ErbB Receptors / genetics
  • Humans
  • Mutation
  • Nanopores*
  • Neoplasms / genetics*
  • Oligonucleotides / chemistry*
  • Polymorphism, Single Nucleotide
  • Proto-Oncogene Proteins p21(ras) / genetics
  • Serogroup
  • Shiga-Toxigenic Escherichia coli / genetics
  • Shiga-Toxigenic Escherichia coli / isolation & purification*

Substances

  • KRAS protein, human
  • Oligonucleotides
  • locked nucleic acid
  • EGFR protein, human
  • ErbB Receptors
  • Proto-Oncogene Proteins p21(ras)