Photobiomodulation modulates the resolution of inflammation during acute lung injury induced by sepsis

Lasers Med Sci. 2019 Feb;34(1):191-199. doi: 10.1007/s10103-018-2688-1. Epub 2018 Nov 15.


Sepsis is a big health problem and one of the most common causes of acute lung injury (ALI) leading to high mortality. Pro-resolving mediators play an important role in abrogating the inflammation and promoting tissue homeostasis restoration. ALI treatment is still a clinical health problem, so new therapies are needed. Here, we evaluated the effect of photobiomodulation treatment on the resolution process of ALI induced by lipopolysaccharide (LPS). Male Balb/c mice were submitted to LPS (ip) or vehicle and irradiated or not with light emitting diode (LED) 2 and 6 h after LPS or vehicle injection, and the parameters were investigated 3 and 7 days after the injections. Our results showed that after 3 days of LED treatment the blood and bronchoalveolar lavage (BAL) cells as well as interleukins (IL) including IL-6 and IL-17 were reduced. No differences were observed in the bone marrow cells, tracheal reactivity, and lipoxin A4 and resolvin E2. Indeed, after 7 days of LED treatment the bone marrow cells, lymphocytes, and lipoxin A4 were increased, while IL-6, IL-17, and IL-10 were decreased. No differences were observed in the blood cells and tracheal reactivity. Thus, our results showed that LED treatment attenuated ALI induced by sepsis by modulating the cell mobilization from their reserve compartments. In addition, we also showed later effects of the LED up to 7 days after the treatment. This study proposes photobiomodulation as therapeutic adjuvant to treat ALI.

Keywords: Acute lung injury; Cytokines; Lipoxins; Photobiomodulation; Resolvins; Tracheal responsiveness.

MeSH terms

  • Acute Lung Injury / etiology*
  • Acute Lung Injury / radiotherapy*
  • Animals
  • Bone Marrow / pathology
  • Bone Marrow / radiation effects
  • Bronchoalveolar Lavage
  • Cell Movement / radiation effects
  • Cholinergic Agents / pharmacology
  • Cytokines / metabolism
  • Eicosapentaenoic Acid / analogs & derivatives
  • Eicosapentaenoic Acid / metabolism
  • Inflammation / pathology
  • Inflammation / radiotherapy*
  • Lipopolysaccharides
  • Lipoxins / metabolism
  • Low-Level Light Therapy*
  • Lung / pathology
  • Lung / radiation effects
  • Male
  • Mice, Inbred BALB C
  • Muscle Contraction / radiation effects
  • Muscle, Smooth / physiopathology
  • Muscle, Smooth / radiation effects
  • Sepsis / complications*


  • Cholinergic Agents
  • Cytokines
  • Lipopolysaccharides
  • Lipoxins
  • lipoxin A4
  • resolvin E2
  • Eicosapentaenoic Acid