Flow Cytometry Instrumentation - An Overview

Curr Protoc Cytom. 2019 Jan;87(1):e52. doi: 10.1002/cpcy.52. Epub 2018 Nov 16.

Abstract

The term flow cytometry, used since the seventies, describes a technology employed mainly in biology and medicine to measure and classify suspended particles, e.g., cells or microspheres. Measurable cell parameters include: geometric properties, such as cell size (diameter, surface area, volume); physiological properties (membrane potential, integrity, vitality); and quantities of DNA, RNA, cytokines, surface antigens, nuclear antigens, enzymes, and proteins. © 2018 by John Wiley & Sons, Inc.

Keywords: detectors; flow cytometry; hydrodynamic focusing; signal processing.

Publication types

  • Review

MeSH terms

  • Flow Cytometry / instrumentation*
  • Fluorescence
  • Humans
  • Signal Processing, Computer-Assisted