Synthesis and biological characterization of monocyte-derived neutrophil chemotactic factor

FEBS Lett. 1988 Aug 29;236(2):467-70. doi: 10.1016/0014-5793(88)80078-4.


MDNCF is a human monocyte-derived, 72-residue chemotactic peptide, which has sequence similarity with members of a family of pro-inflammatory cytokines. The peptide was synthesized by the solid-phase method, and is identical to the natural peptide in amino acid composition, sequence and chemotactic potency. MDNCF forms two loops via a neighboring pair of disulfide bridges, the probable locations of which are residues 7-34 and 9-50. Reduction and alkylation eliminated chemotactic activity. MDNCF fragments 7-37, 30-72 and 17-72 were all biologically inactive. The data suggest that the region of the clustered pair of disulfide bridges is important for biological activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkylation
  • Biological Assay
  • Biological Products / chemical synthesis*
  • Chemotactic Factors / chemical synthesis*
  • Chemotaxis, Leukocyte*
  • Humans
  • Interleukin-8
  • Monokines
  • Neutrophils / physiology
  • Oxidation-Reduction
  • Protein Conformation
  • Structure-Activity Relationship


  • Biological Products
  • Chemotactic Factors
  • Interleukin-8
  • Monokines