Characterization of gastric cells infection by diverse Helicobacter pylori strains through Fourier-transform infrared spectroscopy

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Mar 5;210:193-202. doi: 10.1016/j.saa.2018.11.001. Epub 2018 Nov 5.


The infection of Helicobacter pylori, covering 50% of the world-population, leads to diverse gastric diseases as ulcers and cancer along the life-time of the human host. To promote the discovery of biomarkers of bacterial infection, in the present work, Fourier-transform infrared spectra were acquired from adenocarcinoma gastric cells, incubated with H. pylori strains presenting different genotypes concerning the virulent factors cytotoxin associated gene A and vacuolating cytotoxin A. Defined absorbance ratios were evaluated by diverse methods of statistical inference, according to the fulfillment of the tests assumptions. It was possible to define from the gastric cells, diverse absorbance ratios enabling to discriminate: i) The infection; ii) the bacteria genotype; and iii) the gastric disease of the patients from which the bacteria were isolated. These biomarkers could fasten the knowledge of the complex infection process while promoting a platform for a new diagnostic method, rapid but also specific and sensitive towards the diagnosis of both infection and bacterial virulence.

Keywords: Biomarkers; Gastric adenocarcinoma cells; Helicobacter pylori; Infrared spectroscopy.

MeSH terms

  • Adenocarcinoma / diagnostic imaging
  • Adenocarcinoma / microbiology*
  • Adenocarcinoma / pathology
  • Antigens, Bacterial / genetics
  • Bacterial Proteins / genetics
  • Biomarkers / analysis
  • Genotype
  • Helicobacter Infections / diagnostic imaging*
  • Helicobacter pylori / genetics*
  • Helicobacter pylori / pathogenicity
  • Humans
  • Spectroscopy, Fourier Transform Infrared / methods*
  • Stomach Neoplasms / diagnostic imaging
  • Stomach Neoplasms / microbiology*
  • Stomach Neoplasms / pathology
  • Tumor Cells, Cultured


  • Antigens, Bacterial
  • Bacterial Proteins
  • Biomarkers
  • VacA protein, Helicobacter pylori
  • cagA protein, Helicobacter pylori