MicroRNA-93 promotes bladder cancer proliferation and invasion by targeting PEDF

Urol Oncol. 2019 Feb;37(2):150-157. doi: 10.1016/j.urolonc.2018.08.001. Epub 2018 Nov 17.

Abstract

Objective: MicroRNA-93 (miR-93) is upregulated in the urine of patients with bladder cancer (BC). Here, we investigated the role of miR-93 in BC progression and explored the underlying mechanism.

Methods: miR-93 expression in BC tissues and cells was detected by real time-polymerase chain reaction. The effects of miR-93 and pigment epithelium-derived factor (PEDF) on cell proliferation and invasion were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and Transwell assays. The binding of miR-93 to the 3'-untranslated region of PEDF was identified by the luciferase reporter assay.

Results: miR-93 expression was higher in BC tissues than in normal controls, and its expression was associated with tumor stage and node stage. Inhibition of miR-93 suppressed the proliferation and invasion of BC cells. PEDF was identified as a target of miR-93 and shown to mediate the effect of miR-93 on cell proliferation and invasion.

Conclusions: The present data suggested that miR-93 promoted BC cell proliferation and invasion by targeting PEDF, providing new biomarkers and targets for BC diagnosis and treatment.

Keywords: Bladder cancer; Invasion; Microrna-93; Pigment epithelium-derived factor; Proliferation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomarkers, Tumor / genetics
  • Biomarkers, Tumor / metabolism*
  • Case-Control Studies
  • Cell Movement
  • Cell Proliferation*
  • Eye Proteins / genetics
  • Eye Proteins / metabolism*
  • Female
  • Follow-Up Studies
  • Gene Expression Regulation, Neoplastic*
  • Humans
  • Male
  • MicroRNAs / genetics*
  • Middle Aged
  • Neoplasm Invasiveness
  • Nerve Growth Factors / genetics
  • Nerve Growth Factors / metabolism*
  • Prognosis
  • Serpins / genetics
  • Serpins / metabolism*
  • Tumor Cells, Cultured
  • Urinary Bladder Neoplasms / genetics
  • Urinary Bladder Neoplasms / metabolism
  • Urinary Bladder Neoplasms / pathology*

Substances

  • Biomarkers, Tumor
  • Eye Proteins
  • MIRN93 microRNA, human
  • MicroRNAs
  • Nerve Growth Factors
  • Serpins
  • pigment epithelium-derived factor