Zebrafish Granulocyte Colony-Stimulating Factor Receptor Maintains Neutrophil Number and Function throughout the Life Span

Infect Immun. 2019 Jan 24;87(2):e00793-18. doi: 10.1128/IAI.00793-18. Print 2019 Feb.


Granulocyte colony-stimulating factor receptor (G-CSFR), encoded by the CSF3R gene, represents a major regulator of neutrophil production and function in mammals, with inactivating extracellular mutations identified in a cohort of neutropenia patients unresponsive to G-CSF treatment. This study sought to elucidate the role of the zebrafish G-CSFR by generating mutants harboring these inactivating extracellular mutations using genome editing. Zebrafish csf3r mutants possessed significantly decreased numbers of neutrophils from embryonic to adult stages, which were also functionally compromised, did not respond to G-CSF, and displayed enhanced susceptibility to bacterial infection. The study has identified an important role for the zebrafish G-CSFR in maintaining the number and functionality of neutrophils throughout the life span and created a bona fide zebrafish model of nonresponsive neutropenia.

Keywords: blood diseases; cytokine receptors; developmental hematopoiesis; neutropenia; zebrafish.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Disease Models, Animal
  • Gene Editing
  • Granulocyte Colony-Stimulating Factor
  • Myeloid Cells / cytology
  • Neutropenia / pathology
  • Neutropenia / physiopathology*
  • Neutrophils / cytology
  • Neutrophils / physiology*
  • Receptors, Granulocyte Colony-Stimulating Factor / deficiency
  • Receptors, Granulocyte Colony-Stimulating Factor / physiology*
  • Zebrafish / embryology
  • Zebrafish / physiology*


  • Receptors, Granulocyte Colony-Stimulating Factor
  • Granulocyte Colony-Stimulating Factor