High-resolution crystal structure of parathyroid hormone 1 receptor in complex with a peptide agonist

Nat Struct Mol Biol. 2018 Dec;25(12):1086-1092. doi: 10.1038/s41594-018-0151-4. Epub 2018 Nov 19.


Parathyroid hormone 1 receptor (PTH1R) is a class B multidomain G-protein-coupled receptor (GPCR) that controls calcium homeostasis. Two endogenous peptide ligands, parathyroid hormone (PTH) and parathyroid hormone-related protein (PTHrP), activate the receptor, and their analogs teriparatide and abaloparatide are used in the clinic to increase bone formation as an effective yet costly treatment for osteoporosis. Activation of PTH1R involves binding of the peptide ligand to the receptor extracellular domain (ECD) and transmembrane domain (TMD), a hallmark of class B GPCRs. Here, we present the crystal structure of human PTH1R in complex with a peptide agonist at 2.5-Å resolution, allowing us to delineate the agonist binding mode for this receptor and revealing molecular details within conserved structural motifs that are critical for class B receptor function. Thus, this study provides structural insight into the function of PTH1R and extends our understanding of this therapeutically important class of GPCRs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Biomimetics
  • Crystallography, X-Ray
  • Humans
  • Models, Molecular
  • Parathyroid Hormone / chemistry
  • Peptides / metabolism
  • Protein Binding
  • Receptor, Parathyroid Hormone, Type 1 / chemistry*


  • PTH1R protein, human
  • Parathyroid Hormone
  • Peptides
  • Receptor, Parathyroid Hormone, Type 1