Lactobacillus Plantarum 299V Improves the Microbiological Quality of Legume Sprouts and Effectively Survives in These Carriers During Cold Storage and in Vitro Digestion

PLoS One. 2018 Nov 21;13(11):e0207793. doi: 10.1371/journal.pone.0207793. eCollection 2018.


Probiotics improve consumers' health and additionally may positively influence the microbiological and organoleptic quality of food. In the study, legume sprouts were inoculated with Lactobacilllus plantarum 299V to produce a new functional product ensuring the growth and survival of the probiotic and high microbiological quality of the final product. Legume sprouts, which are an excellent source of nutrients, were proposed as alternative carriers for the probiotic. The key factors influencing the production of probiotic-rich sprouts include the temperature (25°C) of sprouting and methods of inoculation (soaking seeds in a suspension of probiotics). Compared to the control sprouts, the sprouts enriched with the probiotic were characterized by lower mesophilic bacterial counts. In the case of fresh and stored probiotic-rich sprouts, lactic acid bacteria (LAB) accounted for a majority of total microorganisms. The Lb. plantarum population was also stable during the cold storage. The high count of LAB observed in the digest confirmed the fact that the studied sprouts are effective carriers for probiotics and ensure their survival in the harmful conditions of the digestive tract in an in vitro model. Enrichment of legume sprouts with probiotics is a successful attempt and yields products for a new branch of functional foods.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cold Temperature*
  • Digestion*
  • Fabaceae / microbiology*
  • Food Microbiology
  • Food Storage*
  • Lactobacillus plantarum / metabolism
  • Lactobacillus plantarum / physiology*
  • Microbial Viability*
  • Probiotics / metabolism*

Grant support

This work was supported by National Science Centre, Poland (NCN) [Grant OPUS No. 2015/17/B/NZ9/01797 to MŚ]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.