Adipose stem cells and their paracrine factors are therapeutic for early retinal complications of diabetes in the Ins2 Akita mouse

Stem Cell Res Ther. 2018 Nov 21;9(1):322. doi: 10.1186/s13287-018-1059-y.


Background: Early-stage diabetic retinopathy (DR) is characterized by neurovascular defects. In this study, we hypothesized that human adipose-derived stem cells (ASCs) positive for the pericyte marker CD140b, or their secreted paracrine factors, therapeutically rescue early-stage DR features in an Ins2Akita mouse model.

Methods: Ins2Akita mice at 24 weeks of age received intravitreal injections of CD140b-positive ASCs (1000 cells/1 μL) or 20× conditioned media from cytokine-primed ASCs (ASC-CM, 1 μL). Age-matched wildtype mice that received saline served as controls. Visual function experiments and histological analyses were performed 3 weeks post intravitreal injection. Biochemical and molecular analyses assessed the ASC-CM composition and its biological effects.

Results: Three weeks post-injection, Ins2Akita mice that received ASCs had ameliorated decreased b-wave amplitudes and vascular leakage but failed to improve visual acuity, whereas Ins2Akita mice that received ASC-CM demonstrated amelioration of all aforementioned visual deficits. The ASC-CM group demonstrated partial amelioration of retinal GFAP immunoreactivity and DR-related gene expression but the ASC group did not. While Ins2Akita mice that received ASCs exhibited occasional (1 in 8) hemorrhagic retinas, mice that received ASC-CM had no adverse complications. In vitro, ASC-CM protected against TNFα-induced retinal endothelial permeability as measured by transendothelial electrical resistance. Biochemical and molecular analyses demonstrated several anti-inflammatory proteins including TSG-6 being highly expressed in cytokine-primed ASC-CM.

Conclusions: ASCs or their secreted factors mitigate retinal complications of diabetes in the Ins2Akita model. Further investigation is warranted to determine whether ASCs or their secreted factors are safe and effective therapeutic modalities long-term as current locally delivered therapies fail to effectively mitigate the progression of early-stage DR. Nonetheless, our study sheds new light on the therapeutic mechanisms of adult stem cells, with implications for assessing relative risks/benefits of experimental regenerative therapies for vision loss.

Keywords: Adult stem cells; CD140b; MSC; NPDR; TSG-6; Vascular permeability.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / cytology*
  • Adipose Tissue / metabolism
  • Animals
  • Antigens, Surface / chemistry
  • Antigens, Surface / therapeutic use
  • Culture Media, Serum-Free / chemistry
  • Culture Media, Serum-Free / pharmacology
  • Diabetes Mellitus, Experimental / complications*
  • Diabetic Retinopathy / etiology
  • Diabetic Retinopathy / pathology
  • Diabetic Retinopathy / therapy*
  • Humans
  • Intercellular Signaling Peptides and Proteins / metabolism*
  • Intravitreal Injections
  • Male
  • Mesenchymal Stem Cell Transplantation*
  • Mice
  • Mice, Inbred C57BL
  • Thrombomodulin


  • Antigens, Surface
  • Culture Media, Serum-Free
  • Intercellular Signaling Peptides and Proteins
  • THBD protein, human
  • Thrombomodulin