Biosynthesis of cholesterol in the yeast mutant erg6

Biochem Biophys Res Commun. 1988 Aug 30;155(1):509-17. doi: 10.1016/s0006-291x(88)81116-1.

Abstract

A mutant (erg6) of Saccharomyces cerevisiae defective in S-adenosylmethionine: delta 24-sterol-C-methyl transferase (EC2.1.1.41) which normally produces cholesta-5,7,24-trienol and cholesta-5,7,22,24-tetraenol as the major sterols (total 4,4-desmethyl sterol content-8.3 fg/cell) was shown to synthesize trace levels of cholesterol (0.08 fg/cell). The identity of cholesterol was established by co-chromatography in TLC, GLC and HPLC with an authentic sample, mass spectroscopy and after an incubation with [1-14C]acetate by isotopic dilution and recrystallization of the radiochemically purified material to constant specific activity.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Cholestanols / isolation & purification
  • Cholesterol / biosynthesis*
  • Cholesterol / isolation & purification
  • Chromatography, Gel
  • Chromatography, High Pressure Liquid
  • Gas Chromatography-Mass Spectrometry
  • Mutation*
  • S-Adenosylmethionine / deficiency
  • Saccharomyces cerevisiae / analysis
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism*

Substances

  • Cholestanols
  • S-Adenosylmethionine
  • Cholesterol