Biotic elicitation as a tool to improve strawberry and raspberry extract potential on metabolic syndrome-related enzymes in vitro

J Sci Food Agric. 2019 Apr;99(6):2939-2946. doi: 10.1002/jsfa.9507. Epub 2019 Jan 22.


Background: Raspberry and strawberry are high value-added food products that can contribute to human health due to the abundance of polyphenols that they contain. Polyphenols are secondary metabolites and therefore devoted to improve plant adaptation, these polyphenol profile can be induced applying different stimuli, such as certain bacteria. The aim of this study was twofold: (i) to evaluate the ability of two bacterial strains to modulate secondary metabolisms in strawberry and raspberry, and (ii) to explore the ability of plant extracts to modify enzyme activities related to metabolic syndrome.

Results: Total phenolic and anthocyanin content was higher in strawberries than in raspberries, despite similar antioxidant capacities. Strawberry extracts performed better on the tested enzymes, except on α-glucosidase inhibition capacity. Bacillus amyloliquefaciens stabilized the effects of extracts at different points in time, and Pseudomonas fluorescens modified plant metabolism after more inoculations (spring) in both species, improving the effects of raspberry extracts on α-glucosidase, COX1, and COX2, and of strawberry on α-amylase and COX1.

Conclusion: It is good to include these two fruits in the diet because they improve the activity of metabolic syndrome-related enzymes. Applying either strain during plant growth modifies the bioactive profile of the plants, improving the effects of the fruit extracts on human health. © 2018 Society of Chemical Industry.

Keywords: COX2; berries; elicitation; metabolic syndrome; secondary metabolism.

MeSH terms

  • Anthocyanins / chemistry
  • Anthocyanins / metabolism
  • Bacillus amyloliquefaciens / metabolism
  • Cyclooxygenase 1 / chemistry
  • Cyclooxygenase 1 / metabolism
  • Cyclooxygenase 2 / chemistry
  • Cyclooxygenase 2 / metabolism
  • Enzyme Inhibitors / chemistry
  • Enzyme Inhibitors / metabolism
  • Fragaria / metabolism*
  • Fragaria / microbiology
  • Fruit / chemistry
  • Fruit / metabolism
  • Fruit / microbiology*
  • Humans
  • Metabolic Syndrome / diet therapy
  • Metabolic Syndrome / enzymology*
  • Phenols / chemistry
  • Phenols / metabolism
  • Plant Extracts / chemistry*
  • Plant Extracts / metabolism
  • Pseudomonas fluorescens / metabolism
  • Rubus / chemistry
  • Rubus / metabolism*
  • Rubus / microbiology
  • alpha-Amylases / genetics
  • alpha-Amylases / metabolism
  • alpha-Glucosidases / chemistry
  • alpha-Glucosidases / metabolism


  • Anthocyanins
  • Enzyme Inhibitors
  • Phenols
  • Plant Extracts
  • Cyclooxygenase 1
  • Cyclooxygenase 2
  • alpha-Amylases
  • alpha-Glucosidases