PPE65 of M. tuberculosis regulate pro-inflammatory signalling through LRR domains of Toll like receptor-2

Biochem Biophys Res Commun. 2019 Jan 1;508(1):152-158. doi: 10.1016/j.bbrc.2018.11.094. Epub 2018 Nov 22.

Abstract

Our understanding of the PE/PPE family of proteins in M. tuberculosis (Mtb) pathogenesis is still evolving and their critical roles in the host immunomodulation are still in the discovery process. Earlier studies from our group have shown that TLR2-LRR domain plays an important role in regulating cytokine signalling by PPE proteins. The importance of TLR2-LRR domain 16-20 in the regulation of PPE17-induced pro-inflammatory signalling has been established recently. However, it is yet to find whether other PPE protein also targets the TLR2-LRR 16-20 domain for induction of pro-inflammatory responses. In the current study, we have explored the structural parameters and possible role of PPE65 in generating pro-inflammatory signalling molecules mediated through IRAK3 downstream of TLR2-LRR domain 16-20. This study conceptualizes the functional characteristics of PPE65 in infection condition and might possibly provide valuable information in exploring this protein as an immunomodulator in Mtb infection.

Keywords: Cytokines; Homology modeling; Mycobacterium tuberculosis; Pro-inflammatory response; Signalling pathways; TLR2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / chemistry
  • Bacterial Proteins / metabolism*
  • Cloning, Molecular
  • Cytokines / analysis
  • Cytokines / metabolism
  • Inflammation / metabolism*
  • Microscopy, Confocal
  • Models, Molecular
  • Mycobacterium tuberculosis / metabolism*
  • Protein Conformation
  • Protein Domains
  • Signal Transduction*
  • Toll-Like Receptor 2 / chemistry
  • Toll-Like Receptor 2 / metabolism*

Substances

  • Bacterial Proteins
  • Cytokines
  • PPE68 protein, Mycobacterium tuberculosis
  • Toll-Like Receptor 2