Malaria sporozoite penetration. A new approach by double staining

J Immunol Methods. 1988 Sep 13;112(2):201-5. doi: 10.1016/0022-1759(88)90358-4.


To determine, whether a sporozoite is outside the hepatocyte membrane or internalized, a double staining test was carried out using, successively, antibody labeled with peroxidase and fluorescein. This test permits the quantification of sporozoite entry and outline sporozoite-hepatocyte interactions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cells, Cultured
  • Humans
  • Immunoenzyme Techniques
  • Liver / parasitology*
  • Malaria / parasitology*
  • Plasmodium / physiology*