Malaria sporozoite penetration. A new approach by double staining

L Rénia; F Miltgen; Y Charoenvit; T Ponnudurai; J P Verhave; W E Collins; D Mazier

doi:10.1016/0022-1759(88)90358-4

Malaria sporozoite penetration. A new approach by double staining

J Immunol Methods. 1988 Sep 13;112(2):201-5. doi: 10.1016/0022-1759(88)90358-4.

Authors

L Rénia¹, F Miltgen, Y Charoenvit, T Ponnudurai, J P Verhave, W E Collins, D Mazier

Affiliation

¹ INSERM U 313 et Département de Parasitologie, Groupe Hospitalier Pitié-Salpétrière, Paris, France.

PMID: 3047262
DOI: 10.1016/0022-1759(88)90358-4

Abstract

To determine, whether a sporozoite is outside the hepatocyte membrane or internalized, a double staining test was carried out using, successively, antibody labeled with peroxidase and fluorescein. This test permits the quantification of sporozoite entry and outline sporozoite-hepatocyte interactions.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cells, Cultured
Humans
Immunoenzyme Techniques
Liver / parasitology*
Malaria / parasitology*
Plasmodium / physiology*