Effect of Lactobacillus acidophilus and Porphyromonas gingivalis on proliferation and apoptosis of gingival epithelial cells

Adv Med Sci. 2019 Mar;64(1):54-57. doi: 10.1016/j.advms.2018.04.008. Epub 2018 Nov 22.

Abstract

Purpose: This study aimed to evaluate the possible antagonistic effects of Lactobacillus acidophilus on Porphyromonas gingivalis, and detect inhibition of Lactobacillus acidophilus on Porphyromonas gingivalis when they are co-cultured with human gingival epithelial cells.

Materials and methods: Human gingival epithelial cells were co-cultured with Lactobacillus acidophilus and Porphyromonas gingivalis alone or together. The amount of Porphyromonas gingivalis adhering to or invading the epithelial cells were determined by bacterial counts. The cellular proliferation was assayed by the MTT method. Apoptosis was detected by flow cytometry with apoptosis detection kit.

Results: On one hand, Lactobacillus acidophilus reduced the inhibitory effect of Porphyromonas gingivalis on the human gingival epithelial cells proliferation in a dose dependent manner. On the other hand, Porphyromonas gingivalis induced significant apoptosis on human gingival epithelial cells, and Lactobacillus acidophilus inhibited this apoptosis-inducing effect of Porphyromonas gingivalis in a dose dependent manner.

Conclusions: Porphyromonas gingivalis inhibits the proliferation and induces the apoptosis of human gingival epithelial cells. Lactobacillus acidophilus could attenuate this effect in a dose-dependent manner, and it thus reduces the destruction from pathogens. Lactobacillus acidophilus could be an effective candidate for probiotic therapy in periodontal diseases.

Keywords: Human gingival epithelial cell; Lactobacillus acidophilus; Periodontal disease; Porphyromonas gingivalis.

MeSH terms

  • Apoptosis*
  • Cell Proliferation
  • Coculture Techniques
  • Cytoprotection
  • Epithelial Cells / cytology*
  • Epithelial Cells / microbiology*
  • Gingiva / cytology*
  • Humans
  • Lactobacillus acidophilus / physiology*
  • Porphyromonas gingivalis / physiology*