Strain-specific effect on biphasic DNA binding by HIV-1 integrase

AIDS. 2019 Mar 1;33(3):588-592. doi: 10.1097/QAD.0000000000002078.


: The oligomerization of HIV-1 integrase onto DNA is not well understood. Here we show that HIV-1 integrase binds the DNA in biphasic (high-affinity and low-affinity) modes. For HIV-1 subtype B, the high-affinity mode is ∼100-fold greater than the low-affinity mode (Kd.DNA = 37 and 3400 nmol/l, respectively). The Kd.DNA values of patient-derived integrases containing subtype-specific polymorphisms were affected two- to four-fold, suggesting that polymorphisms may have an influence on effective-concentrations of inhibitors, as these inhibitors preferably bind to integrase-DNA complex.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / metabolism*
  • HIV Infections / virology*
  • HIV Integrase / metabolism*
  • HIV-1 / physiology*
  • Humans
  • Kinetics
  • Protein Binding
  • Virus Integration*


  • DNA
  • HIV Integrase
  • p31 integrase protein, Human immunodeficiency virus 1