A Novel Highly Divergent Strain of Cell Fusing Agent Virus (CFAV) in Mosquitoes from the Brazilian Amazon Region

Viruses. 2018 Nov 24;10(12):666. doi: 10.3390/v10120666.


Classical insect-specific flaviviruses (cISFs) have been widely detected in different countries in the last decades. Here, we characterize the near full-length genomes of two cISFs detected in mosquitoes collected in the city of Macapá, state of Amapá, Amazon region of Brazil. A total of 105 pools of female mosquitos were analyzed by next-generation sequencing (NGS). Comparative genomics and phylogenetic analysis identified three strains of cell fusing agent virus (CFAV) and two of Culex flavivirus (CxFV). All sequences were obtained from pools of Culex sp., except for one sequence of CFAV detected in a pool of Aedes aegypti. Both CxFV strains are phylogenetically related to a strain isolated in 2012 in the Southeast region of Brazil. The CFAV strains are the first of this species to be identified in Brazil and one of them is highly divergent from other strains of CFAV that have been detected worldwide. In conclusion, CFAV and CxFV, circulate in mosquitoes in Brazil. One strain of CFAV is highly divergent from others previously described, suggesting that a novel strain of CFAV is present in this region.

Keywords: Amazon region; Culex flavivirus; Culex sp., Aedes aegypti; cell fusing agent virus; flavivirus; mosquitoes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Brazil
  • Culicidae / virology*
  • Evolution, Molecular
  • Flavivirus / classification
  • Flavivirus / physiology*
  • Genome, Viral
  • High-Throughput Nucleotide Sequencing
  • Phylogeny
  • Tropical Climate
  • Viral Nonstructural Proteins / genetics


  • NS5 protein, flavivirus
  • Viral Nonstructural Proteins