Postweaning diarrhea in swine: experimental model of enterotoxigenic Escherichia coli infection

Am J Vet Res. 1988 Jul;49(7):1154-9.


A reproducible model of postweaning colibacillosis was obtained by controlling management and environmental variables to simulate conditions often seen at weaning. Suckling pigs were exposed briefly to starter diet at 1 week of age, weaned at 3 weeks of age, held at an ambient temperature of 20 +/- 2 C, and again given the starter diet. One day after weaning, each pig was given 10(10) colony-forming units of enterotoxigenic Escherichia coli strain M1823B (O157:K88ac:H43-LT+ STb+) in broth containing 1.2% sodium bicarbonate via stomach tube. In vitro adhesion by strain M1823B to isolated intestinal branch borders was used to test pigs for susceptibility to K88. In this model, 3 syndromes were induced in susceptible pigs: (1) peracute fatal diarrhea; (2) moderate diarrhea, weight loss, and fecal shedding of the inoculum strain; and (3) no diarrhea, weight loss, and fecal shedding of the inoculum strain. Rotavirus particles were not found in fecal specimens of pigs with diarrhea. The K88-susceptible, noninoculated control pigs remained clinically normal. It was concluded that susceptibility to adhesion by K88+ enterotoxigenic Escherichia coli was a requirement for the production of disease in this model; inoculation with rotavirus was not necessary.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Animals, Newborn
  • Bacterial Adhesion
  • Diarrhea / microbiology
  • Diarrhea / veterinary*
  • Disease Models, Animal*
  • Escherichia coli / physiology*
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Female
  • Intestinal Mucosa / microbiology
  • Jejunum / microbiology
  • Microvilli / microbiology
  • Swine
  • Swine Diseases / microbiology*
  • Time Factors
  • Weaning