Role of laminin A chain in the development of epithelial cell polarity

Cell. 1988 Oct 21;55(2):331-41. doi: 10.1016/0092-8674(88)90056-6.

Abstract

Kidney organ culture was used to study the conversion of embryonic mesenchymal cells into a polarized, differentiated kidney epithelium. We examined the expression of laminin, a basement membrane glycoprotein, during this conversion. The B chains of laminin were constitutively expressed, whereas the appearance of the A chain of laminin was dependent on embryonic induction and coincided with the onset of cell polarization. Antisera against the carboxy-terminal end of laminin inhibited polarization but did not affect the developmental events that precede polarization. Antisera against N-terminal parts of laminin failed to inhibit morphogenesis. Since the fragments at the carboxy-terminal end contain parts of the A chain, we suggest that the appearance of this chain is fundamental for initiation of cell polarity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Culture Techniques
  • Epithelial Cells
  • Fluorescent Antibody Technique
  • Kidney / cytology*
  • Laminin / physiology*
  • Macromolecular Substances
  • Mice
  • Morphogenesis
  • Structure-Activity Relationship

Substances

  • Laminin
  • Macromolecular Substances