Expression of Genes Encoding Cell Motility Proteins during Progression of Head and Neck Squamous Cell Carcinoma

G V Kakurina; I V Kondakova; L V Spirina; E S Kolegova; E E Shashova; O V Cheremisina; V A Novikov; E L Choinzonov

doi:10.1007/s10517-018-4325-1

Expression of Genes Encoding Cell Motility Proteins during Progression of Head and Neck Squamous Cell Carcinoma

Bull Exp Biol Med. 2018 Dec;166(2):250-252. doi: 10.1007/s10517-018-4325-1. Epub 2018 Nov 28.

Authors

G V Kakurina¹, I V Kondakova², L V Spirina^{2

3}, E S Kolegova², E E Shashova², O V Cheremisina², V A Novikov², E L Choinzonov^{2

3}

Affiliations

¹ Research Institute of Oncology, Tomsk National Research Medical Center, Russian Academy of Sciences, Moscow, Russia. kakurinagv@oncology.tomsk.ru.
² Research Institute of Oncology, Tomsk National Research Medical Center, Russian Academy of Sciences, Moscow, Russia.
³ Siberian State Medical University, Tomsk, Russia.

PMID: 30488206
DOI: 10.1007/s10517-018-4325-1

Abstract

The model of head and neck squamous cell carcinoma (HNSCC) was used to study the expression of genes encoding actin-binding proteins depending on the type of cell motility. The expression of SNAIL1 and CAPN2 mRNA in HNSCC tissue was higher than in specimens of dysplastic epithelium of the larynx and hypopharynx, which can be explained by activation of mesenchymal and amoeboid types of cell motility. In biopsy material of HNSCC patients with T1-2N0M0, expression of genes responsible for actin-binding proteins differed from that of patients with pretumor pathology of the larynx and hypopharynx: expression of FSCN was lower, while expressions of EZR and CAP1 were higher. The data attest that progression of HNSCC is associated with activation of both types of cell motility and with the changes in the expression of mRNA encoding cell motility proteins.

Keywords: actin-binding proteins; chronic hyperplastic laryngitis; head and neck squamous cell carcinoma; locomotion; mesenchymal and amoeboid types of cell motility.

MeSH terms

Calpain / genetics*
Calpain / metabolism
Carrier Proteins / genetics
Carrier Proteins / metabolism
Cell Cycle Proteins / genetics
Cell Cycle Proteins / metabolism
Cell Line, Tumor
Cell Movement / genetics*
Cofilin 1 / genetics
Cofilin 1 / metabolism
Cytoskeletal Proteins / genetics
Cytoskeletal Proteins / metabolism
Disease Progression
Female
Gene Expression Regulation, Neoplastic*
Head and Neck Neoplasms / genetics*
Head and Neck Neoplasms / metabolism
Head and Neck Neoplasms / pathology
Humans
Hypopharynx / metabolism
Hypopharynx / pathology
Larynx / metabolism
Larynx / pathology
Male
Microfilament Proteins / genetics
Microfilament Proteins / metabolism
Middle Aged
Profilins / genetics
Profilins / metabolism
Signal Transduction
Snail Family Transcription Factors / genetics*
Snail Family Transcription Factors / metabolism
Squamous Cell Carcinoma of Head and Neck / genetics*
Squamous Cell Carcinoma of Head and Neck / metabolism
Squamous Cell Carcinoma of Head and Neck / pathology
Vimentin / genetics
Vimentin / metabolism
rho GTP-Binding Proteins / genetics
rho GTP-Binding Proteins / metabolism

Substances

CAP1 protein, human
CFL1 protein, human
Carrier Proteins
Cell Cycle Proteins
Cofilin 1
Cytoskeletal Proteins
FSCN1 protein, human
Microfilament Proteins
PFN1 protein, human
Profilins
SNAI1 protein, human
Snail Family Transcription Factors
VIM protein, human
Vimentin
ezrin
Calpain
CAPN2 protein, human
RND3 protein, human
rho GTP-Binding Proteins