Vicenin-2 acts as a radiosensitizer of the non-small cell lung cancer by lowering Akt expression

Biofactors. 2019 Mar;45(2):200-210. doi: 10.1002/biof.1472. Epub 2018 Nov 29.

Abstract

Non-small cell lung cancer (NSCLC) has a very high rate of incidence and is resistant to chemo- and radiotherapy. Vicenin-2 (VCN-2) is a flavonoid obtained from Ocimum sanctum L. and it has been reported to have radioprotective, anticancer, and radiosensitizing properties. We have conducted this study to check the effect of VCN-2 on the cell viability and the effect on PTEN (Phosphatase and tensin homolog), PI3KCA (Phosphatidylinositol 4, 5-biphosphate 3-kinase catalytic subunit alpha isoform/PI3K 110α subunit), and Akt1 when VCN-2 was used alone and in combination with radiation in the NSCLC cell line NCI-H23 (H23). We have also checked the effect of VCN-2 on various pro- and anti-apoptotic genes and the ultra-morphological changes that occurred in the cells when VCN-2 is used alone and in combination with radiation. VCN-2 was able to lower cancer cell survival and phosphorylated Akt while promoting the expression of pro-apoptotic genes and down-regulating anti-apoptotic genes. We also observed the apoptosis-associated ultra-morphological changes in the VCN-2-treated cells. Our study have demonstrated that VCN-2 can be a potential chemotherapeutic and radiosensitizing agent in NSCLC. © 2018 BioFactors, 45(2):200-210, 2019.

Keywords: Akt; PI3K; PTEN; Vicenin-2; apoptosis; non-small cell lung cancer; radiosensitization.

MeSH terms

  • Apigenin / pharmacology*
  • Apoptosis / drug effects
  • Blotting, Western
  • Carcinoma, Non-Small-Cell Lung / metabolism*
  • Cell Line, Tumor
  • Cell Survival / drug effects
  • Chromones / pharmacology
  • Glucosides / pharmacology*
  • Humans
  • Lung Neoplasms / metabolism*
  • Morpholines / pharmacology
  • Nuclear Proteins / genetics
  • Nuclear Proteins / metabolism
  • Ocimum sanctum / chemistry
  • PTEN Phosphohydrolase / genetics
  • PTEN Phosphohydrolase / metabolism
  • Protein Carbonylation / drug effects
  • Proto-Oncogene Proteins c-akt / metabolism*
  • Radiation-Sensitizing Agents / pharmacology*
  • Reactive Oxygen Species / metabolism
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Chromones
  • Glucosides
  • Morpholines
  • Nuclear Proteins
  • PI3KCA protein, human
  • Radiation-Sensitizing Agents
  • Reactive Oxygen Species
  • Transcription Factors
  • apigenin-6,8-di-C-glycopyranoside
  • 2-(4-morpholinyl)-8-phenyl-4H-1-benzopyran-4-one
  • Apigenin
  • Proto-Oncogene Proteins c-akt
  • PTEN Phosphohydrolase