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. 2019 Feb 1;174:459-466.
doi: 10.1016/j.colsurfb.2018.11.052. Epub 2018 Nov 22.

Production of a Microbial Emulsifier With Biotechnological Potential for Environmental Applications

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Production of a Microbial Emulsifier With Biotechnological Potential for Environmental Applications

Macarena María Rulli et al. Colloids Surf B Biointerfaces. .

Abstract

The present work reports the production of bioemulsifiers (BEs) by an environmental bacterium closely related to Bacillus spp., using agro-industrial wastes and by-products as low-cost carbon sources. Maximum emulsifying activity was detected using crude glycerol (CG) (E24 = 59%), producing 2.8 g/L of BE at 24 h of incubation. The microbial product presented significantly higher biodegradability in comparison to three commercial emulsifying agents (sodium dodecyl sulfate, SDS; Tween 80; and Arabic gum, AG). Interestingly, BE proved to be innocuous for Caco-2 cells and wheat seedlings, used as toxicological indicators. The BE and AG showed (in most of the cases) higher stability to changes in temperature (37 °C-100 °C), pH (3-10), and salt concentration (5% and 10%, w/v) in comparison to the SDS and Tween 80. Finally, the microbial product displayed a large affinity to a wide range of hydrophobic substrates showing emulsifying activities similar to or even better than SDS, Tween 80 and AG. The results presented in this study demonstrate the potential of a bioproduct obtained from CG to be used for environmental purposes.

Keywords: Bacillus sp.; Biodegradability; Bioemulsifiers; Caco-2 cells; Crude glycerol; Hydrocarbons; Organochlorine pesticides; Triticum aestivum L.; Waste oils.

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