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, 12 (4), 483-491
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Karyotype and Chromosomal Characteristics of rDNA of Cobitisstrumicae Karaman, 1955 (Teleostei, Cobitidae) From Lake Volvi, Greece

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Karyotype and Chromosomal Characteristics of rDNA of Cobitisstrumicae Karaman, 1955 (Teleostei, Cobitidae) From Lake Volvi, Greece

Eva Hnátková et al. Comp Cytogenet.

Abstract

The karyotype of Greek cobitid fish Cobitisstrumicae Karaman, 1955, from Lake Volvi, Greece, a representative of one of its two major intraspecific phylogenetic lineages, was analysed by means of sequential Giemsa-staining, C-banding, silver-staining, CMA3 fluorescence banding and also by in situ hybridization (FISH) with rDNA probe. The diploid chromosome number was 2n = 50, karyotype composed of 10 pairs of metacentric to submetacentric and 15 pairs of subtelocentric to acrocentric chromosomes. The nucleolus organizer regions (NORs) as revealed by Ag- and CMA3 staining and FISH were situated in the telomeric region of the fourth submetacentric chromosome pair. The chromosomes contained very low content of C-positive heterochromatin. No heteromorphic sex chromosomes were detected. This first karyotype report for any species of lineage Bicanestrinia Băcescu, 1962 shows a simple karyotype dominated by acrocentric chromosomes and possessing single NOR-bearing chromosome pair. Cytotaxonomic implications of this finding for the taxonomy of the genus Cobitis Linnaeus, 1758 are further discussed.

Keywords: FISH; NOR phenotype; chromosome banding; cytotaxonomy of Cobitis loaches; rDNA.

Figures

Figure 1.
Figure 1.
Karyotypes of a male (a) and female (b) of C.strumicae from Lake Volvi arranged from sequentially Giemsa-stained (upper row) and C-banded (lower row) chromosomes; sequentially Ag-stained chromosome pair with positive signal is framed in (a) and (b); metaphase cells of C.strumicae after CMA3 staining (c) and FISH with rDNA probe (d) chromosomes bearing CMA3 and FISH signals are framed; m – metacentric, sm – submetacentric, st – subtelocentric and a – acrocentric chromosomes. Scale bar: 10 μm.

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