Efficient synthesis and characterisation of the amyloid beta peptide, Aβ 1-42, using a double linker system

Org Biomol Chem. 2018 Dec 19;17(1):30-34. doi: 10.1039/c8ob02929f.

Abstract

The amyloidogenic Aβ42 peptide was efficiently prepared using a double linker system, markedly improving solubility and chromatographic peak resolution, thus enabling full characterisation using standard techniques. The tag was readily cleaved with sodium hydroxide and removed by aqueous extraction, affording Aβ42 in high purity and yield for biophysical characterisation studies.

MeSH terms

  • Amyloid beta-Peptides / chemical synthesis*
  • Chromatography, High Pressure Liquid
  • Humans
  • Liquid-Liquid Extraction
  • Peptide Fragments / chemical synthesis*
  • Sodium Hydroxide / chemistry
  • Solubility
  • Staining and Labeling / methods*

Substances

  • Amyloid beta-Peptides
  • Peptide Fragments
  • amyloid beta-protein (1-42)
  • Sodium Hydroxide