A New Tool for Inducible Gene Expression in Caenorhabditis elegans

Genetics. 2019 Feb;211(2):419-430. doi: 10.1534/genetics.118.301705. Epub 2018 Nov 30.


Controlling protein activity and localization is a key tool in modern biology. Mammalian steroid receptor ligand-binding domain (LBD) fusions have been used in a range of organisms and cell types to inactivate proteins of interest until the cognate steroid ligand is applied. Here, we demonstrate that the glucocorticoid receptor LBD confers ligand-gated control of a heterologous gene expression system (Q system) and the DAF-16 transcription factor in Caenorhabditis elegans These experiments provide a powerful tool for temporal control of protein activity, and will bolster existing tools used to modulate gene expression and protein activity in this animal.

Keywords: Caenorhabditis elegans; Glucocorticoid receptor; drug inducible; gene expression; ligand-binding domain.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism
  • Caenorhabditis elegans Proteins / genetics
  • Caenorhabditis elegans Proteins / metabolism
  • Dexamethasone / pharmacology
  • Forkhead Transcription Factors / genetics
  • Forkhead Transcription Factors / metabolism
  • Genetic Techniques*
  • Glucocorticoids / pharmacology
  • Humans
  • Receptors, Glucocorticoid / genetics
  • Receptors, Glucocorticoid / metabolism
  • Transcriptional Activation*


  • Caenorhabditis elegans Proteins
  • Forkhead Transcription Factors
  • Glucocorticoids
  • Receptors, Glucocorticoid
  • daf-16 protein, C elegans
  • Dexamethasone

Associated data

  • figshare/10.25386/genetics.7390391