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. 2018 Nov 5;8(21):e3073.
doi: 10.21769/BioProtoc.3073.

Generation of Stable Expression Mammalian Cell Lines Using Lentivirus

Neha Tandon  1 , Kaushik N Thakkar  2 , Edward L LaGory  2 , Yu Liu  1 , Amato J Giaccia  2
Affiliations

Generation of Stable Expression Mammalian Cell Lines Using Lentivirus

Neha Tandon et al. Bio Protoc. .

Abstract

Lentiviruses are used very widely to generate stable expression mammalian cell lines. They are used for both gene down-regulation (by using shRNA) or for gene up-regulation (by using ORF of gene of interest). The technique of generating stable cell lines using 3rd generation lentivirus is very robust and it typically takes about 1-2 weeks to get stable expression for most mammalian cell lines. The advantage of using the 3rd generation lentivirus are that are very safe and they are replication incompetent.

Keywords: Gene knockdown; Gene over-expression; Lentivirus; Mammalian cell lines; Stable cell lines; Transduction.

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Conflict of interest statement

Competing interests The authors declare no conflicts of interest or competing interests.

Figures

Figure 1.
Figure 1.. Schematic representation of generation of stable cell lines using lentivirus
See this image and copyright information in PMC

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References

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