Toxicity of food sweetener-sodium cyclamate on osteoblasts cells

Biochem Biophys Res Commun. 2019 Jan 8;508(2):507-511. doi: 10.1016/j.bbrc.2018.11.172. Epub 2018 Nov 30.


In this paper, the effect of commonly used food sweetener (sodium cyclamate) on the proliferation and differentiation of osteoblasts has been researched. The morophology change of osteoblasts was investigated by confocal laser scanning microscopy. Cell viability was studied by MTT analysis. BMP2 expression was analyzed by western blot and immunofluorescence. Mineralization ability of osteoblasts was researched by using alizarin red staining method. The results indicate that a very low concentration (0.06 μM) of sodium cyclamate can curle and fold microfilament and microtubule of osteoblasts. The increase addition of sodium cyclamate resulted significantly decrease of cells viability. The expression of bone morphogenetic protein-2 (BMP2) was seriously suppressed by sodium cyclamate. Alizarin Red staining experiment revealed that sodium cyclamate decreased the mineralization ability of osteoblasts. The present results suggest that sodium cyclamate can seriously inhibit the proliferation and differentiation of osteoblasts.

Keywords: Food safety; Osteoporosis; Sodium cyclamate; Sweetener.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bone Morphogenetic Protein 2 / drug effects
  • Calcification, Physiologic / drug effects
  • Cell Differentiation / drug effects
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects
  • Cyclamates / toxicity*
  • Osteoblasts / cytology
  • Osteoblasts / drug effects*
  • Sweetening Agents / toxicity*


  • BMP2 protein, human
  • Bone Morphogenetic Protein 2
  • Cyclamates
  • Sweetening Agents