Significant association of TOX3/LOC643714 locus-rs3803662 and breast cancer risk in a cohort of Iranian population

Mol Biol Rep. 2019 Feb;46(1):805-811. doi: 10.1007/s11033-018-4535-7. Epub 2018 Dec 4.


Genome-wide association studies normally focus on low penetrance and moderate to high-frequency single nucleotide polymorphisms (SNPs), which lead to genetic susceptibility to breast cancer. In this regard, the T allele of rs3803662 has been associated with breast cancer risk and with lower expression level of TOX3. We aimed to assess the risk of breast cancer associated with this polymorphism in an Iranian population. Using Tetra Primer ARMS PCR, rs3803662 was analyzed in a total of 943 individuals (430 cases and 513 healthy controls form North East of Iran). Allele frequencies and genotype distribution were analyzed in case and control samples to find out any association using the Chi-squared test and Logistic regression. All cases were pathologically confirmed; all controls were mainly healthy individuals. Genotype frequencies were found to be in agreement with HWE in controls and cases. TOX3-rs3803662 SNP was associated with breast cancer risk in our study (T vs. C allele contrast model: OR 1.36, 95% CI 1.12-1.64, Pvalue = 0.002; TT vs. CT + TT dominant model: OR 0.67, 95% CI 0.51-0.87, Pvalue = 0.003; TT vs. CT + CC recessive model: OR 1.54, 95% CI 1.02-2.30, Pvlue = 0.036). Moreover, after adjusting for age, BMI, history of previous cancer and also family history of cancer, all results, except for the recessive model, were remained significant. TOX3-rs3803662, may confer some degrees of risk of breast cancer in Iranian population. This finding is in line with similar results in other populations. It highlights the importance of TOX3 pathway in tumorigenesis.

Keywords: Breast carcinoma; Cancer risk; Outcomes; TOX3/ TNRC9.

MeSH terms

  • Apoptosis Regulatory Proteins
  • Breast Neoplasms / genetics*
  • Case-Control Studies
  • Cohort Studies
  • Female
  • Genetic Loci*
  • Genetic Predisposition to Disease*
  • Genome-Wide Association Study*
  • High Mobility Group Proteins
  • Humans
  • Iran
  • Middle Aged
  • Polymorphism, Single Nucleotide / genetics*
  • Receptors, Progesterone / genetics*
  • Risk Factors
  • Trans-Activators


  • Apoptosis Regulatory Proteins
  • High Mobility Group Proteins
  • Receptors, Progesterone
  • TOX3 protein, human
  • Trans-Activators