HelixComplex snail mucus exhibits pro-survival, proliferative and pro-migration effects on mammalian fibroblasts

Sci Rep. 2018 Dec 5;8(1):17665. doi: 10.1038/s41598-018-35816-3.

Abstract

Snail mucus is a mixture of active substances commonly thought to have healthy properties for the treatment of skin disorders. Although snail mucus is an ingredient of several cosmetic and para-pharmaceutic products, a comprehensive characterization of chemical composition and biological effects is still missing. Crude purified extracts from Helix aspersa muller mucus (HelixComplex) were prepared and, after chemical characterization, tested on in vitro experimental models. Differently from what expected, HelixComplex was characterized by the presence of small amounts of glycolic acid and allantoin. By using different in vitro assays on fibroblast cultures, we found that HelixComplex lacked of cytotoxicity, protected cells from apoptosis (p < 0.05) and, importantly, was able to significantly induce cell proliferation and migration through direct and indirect mechanisms. These effects were associated to morphological changes, cytoskeleton re-organization and release of cytokines. In conclusion, our findings suggest that snail mucus biological effects are attributable to cell proliferation and migration, and pave the way for further investigating snail mucus potential as therapeutic agent.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Allantoin / analysis
  • Allantoin / pharmacology
  • Animals
  • Apoptosis / drug effects
  • Cell Line
  • Cell Movement* / drug effects
  • Cell Proliferation* / drug effects
  • Cell Survival / drug effects
  • Cytoprotection / drug effects
  • Fibroblasts / cytology*
  • Fibroblasts / drug effects
  • Glycolates / analysis
  • Glycolates / pharmacology
  • Helix, Snails / chemistry*
  • Helix, Snails / microbiology
  • Humans
  • Mice
  • Mucus / chemistry*
  • Mucus / microbiology
  • NIH 3T3 Cells
  • Wound Healing / drug effects

Substances

  • Glycolates
  • glycolic acid
  • Allantoin