Controlling the mitochondrial antisense - role of the SUV3-PNPase complex and its co-factor GRSF1 in mitochondrial RNA surveillance

Zbigniew Pietras; Magdalena A Wojcik; Lukasz S Borowski; Maciej Szewczyk; Tomasz M Kulinski; Dominik Cysewski; Piotr P Stepien; Andrzej Dziembowski; Roman J Szczesny

doi:10.1080/23723556.2018.1516452

Controlling the mitochondrial antisense - role of the SUV3-PNPase complex and its co-factor GRSF1 in mitochondrial RNA surveillance

Mol Cell Oncol. 2018 Sep 20;5(6):e1516452. doi: 10.1080/23723556.2018.1516452. eCollection 2018.

Authors

Zbigniew Pietras^{1

2}, Magdalena A Wojcik^{1

3}, Lukasz S Borowski^{1

3}, Maciej Szewczyk^{1

3}, Tomasz M Kulinski¹, Dominik Cysewski¹, Piotr P Stepien^{1

3}, Andrzej Dziembowski^{1

3}, Roman J Szczesny¹

Affiliations

¹ Laboratory of RNA Biology and Functional Genomics, Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland.
² Laboratory of Protein Structure, International Institute of Molecular and Cell Biology, Warsaw, Poland.
³ Faculty of Biology, Institute of Genetics and Biotechnology, University of Warsaw, Warsaw, Poland.

Abstract

Transcription of the human mitochondrial genome produces a vast amount of non-coding antisense RNAs. These RNA species can form G-quadraplexes (G4), which affect their decay. We found that the mitochondrial degradosome, a complex of RNA helicase SUPV3L1 (best known as SUV3) and the ribonuclease PNPT1 (also known as PNPase), together with G4-melting protein GRSF1, is a key player in restricting antisense mtRNAs.

Keywords: G-quadraplexes; G4; GRSF1; PNPase; RNA surveillance; SUV3; mitochondrial RNA.

Grants and funding

This work was supported by the National Science Centre, Poland under Grants UMO-2014/12/W/NZ1/00463 (to R.J.S.), 2013/11/B/NZ1/00089 (to P.P.S.); European Research Council under Starting Grant 309419 PAPs & PUPs (to A.D.).