Effects of Lactobacillus salivarius WB21 combined with green tea catechins on dental caries, periodontitis, and oral malodor

Arch Oral Biol. 2019 Feb;98:243-247. doi: 10.1016/j.archoralbio.2018.11.027. Epub 2018 Nov 30.


Objective: To evaluate the combined use of Lactobacillus salivarius WB21 and (-)-epigallocatechin gallate (EGCg) for oral health maintenance.

Design: The effects of L. salivarius WB21 on growth of Streptococcus mutans, the insoluble glucan produced by S. mutans, and on growth of Porphyromonas gingivalis were evaluated in vitro. In addition, the susceptibility of five oral pathogenic bacteria and L. salivarius WB21 to EGCg, the inhibiting effect of EGCg on methyl mercaptan, and the effects of L. salivarius WB21 and EGCg in combination on growth of P. gingivalis were examined.

Results: Lactobacillus salivarius WB21 showed concentration-dependent inhibition of the growth of S. mutans. Addition of L. salivarius WB21 inhibited production of the insoluble glucan by S. mutans (p < 0.001). A filtrate of L. salivarius WB21 culture solution inhibited growth of P. gingivalis (p < 0.001 vs. control), and this effect was enhanced when it was used in combination with EGCg (p < 0.001 vs. the addition of L. salivarius WB21). In addition, EGCg directly inhibited methyl mercaptan in a concentration-dependent manner (p < 0.001). Concerning bacterial susceptibility to EGCg, growth of P. gingivalis, Prevotella intermedia, and Fusobacterium nucleatum was inhibited at 2.5 mg/mL of EGCg, while that of L. salivarius WB21 was inhibited at 25 mg/mL EGCg.

Conclusions: Our results imply that L. salivarius WB21 may be useful for controlling dental caries, periodontitis, and oral malodor. In addition, the effects of L. salivarius WB21 on periodontitis and oral malodor may be synergistically enhanced by use in combination with EGCg.

Keywords: (–)-Epigallocatechin gallate; Dental caries; Lactobacillus salivarius; Oral malodor; Periodontitis.

MeSH terms

  • Antibiosis
  • Catechin / analogs & derivatives
  • Catechin / pharmacology*
  • Catechin / physiology
  • Dental Caries / microbiology*
  • Dental Caries / prevention & control
  • Fusobacterium nucleatum / drug effects
  • Fusobacterium nucleatum / growth & development
  • Glucans / metabolism
  • Halitosis / microbiology*
  • Halitosis / prevention & control
  • Lactobacillus salivarius / drug effects
  • Lactobacillus salivarius / physiology*
  • Microbial Sensitivity Tests
  • Periodontitis / microbiology*
  • Periodontitis / prevention & control
  • Porphyromonas gingivalis / drug effects
  • Porphyromonas gingivalis / growth & development
  • Prevotella intermedia / drug effects
  • Prevotella intermedia / growth & development
  • Probiotics
  • Streptococcus mutans / drug effects
  • Streptococcus mutans / growth & development
  • Streptococcus mutans / metabolism
  • Tea / chemistry*


  • Glucans
  • Tea
  • Catechin
  • epigallocatechin gallate