Cloning a synthetic gene for human stefin B and its expression in E. coli

FEBS Lett. 1988 Oct 24;239(1):41-4. doi: 10.1016/0014-5793(88)80541-6.


A gene coding for human stefin B was synthesized by the solid-phase phosphite method and cloned in the pUC8 cloning vector. The insert with the verified DNA sequence was subcloned into two expression vectors and expressed in E. coli as a fusion protein with beta-galactosidase and as a native protein. The CNBr cleaved fusion protein and the native recombinant stefin B were inhibitory to papain and reacted with antibodies against human stefin B.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular*
  • Cystatin B
  • Cystatins*
  • Escherichia coli / genetics*
  • Genes*
  • Genes, Synthetic*
  • Humans
  • Molecular Sequence Data
  • Protease Inhibitors / genetics*
  • Proteins / genetics*
  • Proteins / isolation & purification
  • Recombinant Proteins / isolation & purification
  • Transcription, Genetic


  • CSTB protein, human
  • Cystatins
  • Protease Inhibitors
  • Proteins
  • Recombinant Proteins
  • Cystatin B