Methods to Probe Calcium Regulation by BCL-2 Family Members

Methods Mol Biol. 2019:1877:173-183. doi: 10.1007/978-1-4939-8861-7_12.

Abstract

BCL-2 family members have additional roles beyond direct regulation of mitochondrial outer membrane permeabilization (MOMP) in apoptosis. One such important function is the release of calcium from the endoplasmic reticulum (ER), which critically contributes to the process of apoptosis. Here, we describe a protocol to measure calcium levels in the ER, mitochondria, and cytosol, with specific consideration of BCL-2 family biology.

Keywords: Apoptosis; BCL-2 Family; Calcium; Endoplasmic reticulum; Mitochondria.

MeSH terms

  • Animals
  • Apoptosis / physiology
  • Biological Transport / physiology
  • Calcium / metabolism*
  • Cytosol / metabolism
  • Endoplasmic Reticulum / metabolism
  • Humans
  • Mitochondria / metabolism
  • Mitochondrial Membranes / metabolism
  • Permeability
  • Proto-Oncogene Proteins c-bcl-2 / metabolism*

Substances

  • Proto-Oncogene Proteins c-bcl-2
  • Calcium