RNA-aptamers-As targeted inhibitors of protein functions in plants

J Plant Physiol. 2019 Jan;232:127-129. doi: 10.1016/j.jplph.2018.10.026. Epub 2018 Nov 3.

Abstract

The scope of RNA-aptamers application is becoming wider and has expanded beyond solely medical use. We propose the use of RNA-aptamers in plants to suppress the functions of individual proteins, thereby achieving resistance to various biotic and abiotic stresses. In current work we experimentally demonstrate the possibility of inhibiting protein activity in N. bentamiana plants by quenching the fluorescence level of GFP (green fluorescent protein) as a result of specifically selected RNA-aptamer binding action.

Keywords: GFP protein; Inhibition of proteins activity; RNA-aptamers.

MeSH terms

  • Aptamers, Nucleotide / metabolism*
  • Green Fluorescent Proteins / metabolism
  • Plant Proteins / metabolism*
  • Plant Proteins / physiology
  • Tobacco / metabolism

Substances

  • Aptamers, Nucleotide
  • Plant Proteins
  • Green Fluorescent Proteins