Recognition of malignant melanoma by monoclonal antibody HMB-45. An immunohistochemical study of 200 paraffin-embedded cutaneous tumors

J Cutan Pathol. 1988 Aug;15(4):201-7. doi: 10.1111/j.1600-0560.1988.tb00544.x.


Antibodies to S-100 protein have been used widely as markers of malignant melanoma, despite abundant evidence that they are non-specific for this neoplasm. Hence, alternatives to these reagents are desirable in diagnostic dermatopathology. We evaluated the characteristics of a new monoclonal antibody (HMB-45) which does have putative specificity for melanoma, and compared it with a polyclonal anti-S-100 reagent in immunohistochemical staining of 67 melanomas of the skin and 133 non-melanomatous cutaneous neoplasms. All specimens were formalin-fixed and paraffin-embedded, and were studied with the avidin-biotin-peroxidase complex technique. HMB-45 labelled 62 of 67 melanomas, while anti-S-100 recognized all tumors of this type. On the other hand, S-100 also was expressed by 15 of the non-melanocytic neoplasms, all 133 of which were HMB-45-negative. The only cases of melanoma that were missed by the latter reagent were of the spindle-cell type. Hence, HMB-45 was 100% specific and 93% sensitive, relative to a diagnosis of malignant melanoma in paraffin sections. Epithelioid and small-cell neoplasms are reliably recognized by this antibody, but it would appear that spindle-cell melanomas must be detected by other immunohistochemical means.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies, Monoclonal*
  • Antigens, Neoplasm / analysis*
  • Antigens, Surface / analysis
  • Humans
  • Hybridomas / immunology
  • Immunoenzyme Techniques
  • Immunohistochemistry
  • Melanoma / immunology
  • Melanoma / pathology*
  • S100 Proteins / immunology
  • Skin Neoplasms / immunology
  • Skin Neoplasms / pathology*


  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Antigens, Surface
  • S100 Proteins