Cystatin C in adipose tissue and stimulation of its production by growth hormone and triiodothyronine in 3T3-L1 cells

Mol Cell Endocrinol. 2019 Feb 15:482:28-36. doi: 10.1016/j.mce.2018.12.004. Epub 2018 Dec 10.

Abstract

Cystatin C (CysC) is a marker for estimation of glomerular filtration rate (GFR). CysC levels may depend not only on clearance/GFR but possibly also on changes in production. Our studies on tissue distribution of CysC protein in mice showed that adipose tissue expresses significant amounts of CysC, suggesting that adipocytes could contribute to circulating CysC levels in vivo. As growth hormone (GH) and triiodothyronine (T3) increase both GFR and CysC (increased in acromegaly and hyperthyroidism) in vivo, we studied whether they could increase CysC production in 3T3-L1 adipocytes in vitro. CysC accumulated in culture media of 3T3-L1 adipocytes in a time-dependent fashion. GH and T3 both (10 nmol/l) increased accumulation of CysC, to 373 ± 14 and 422 ± 20, respectively, vs 298 ± 10 ng per well over 4 days in controls. Thus, GH and T3 enhance the production of CysC by adipocytes in vitro.

Keywords: 3T3-L1; Adipocytes; Cystatin C; Growth hormone; Mouse; Triiodothyronine.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3-L1 Cells
  • Adipose Tissue / drug effects
  • Adipose Tissue / metabolism*
  • Animals
  • Cystatin C / genetics
  • Cystatin C / metabolism*
  • Gene Expression Regulation / drug effects
  • Glomerular Filtration Rate / drug effects
  • Growth Hormone / pharmacology*
  • Mice
  • Time Factors
  • Triiodothyronine / pharmacology*

Substances

  • Cystatin C
  • Triiodothyronine
  • Growth Hormone