Gene editing technologies and applications for insects

Curr Opin Insect Sci. 2018 Aug;28:66-72. doi: 10.1016/j.cois.2018.05.006. Epub 2018 May 22.

Abstract

Initially discovered in bacteria, CRISPR-based genome editing endonucleases have proven remarkably amenable for adaptation to insects. To date, these endonucleases have been utilized in a plethora of both model and non-model insects including diverse flies, bees, beetles, butterflies, moths, and grasshoppers, to name a few, thereby revolutionizing functional genomics of insects. In addition to basic genome editing, they have also been invaluable for advanced genome engineering and synthetic biology applications. Here we explore the recent genome editing advancements in insects for generating site-specific genomic mutations, insertions, deletions, as well as more advanced applications such as Homology Assisted Genome Knock-in (HACK), potential to utilize DNA base editing, generating predictable reciprocal chromosomal translocations, and development gene drives to control the fate of wild populations.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Review

MeSH terms

  • Animals
  • Gene Deletion
  • Gene Drive Technology / methods
  • Gene Editing / instrumentation
  • Gene Editing / methods*
  • Gene Knock-In Techniques / methods
  • Genome*
  • Insecta / genetics*
  • Mutagenesis, Insertional
  • Mutation
  • Translocation, Genetic