Allergy inhibitory receptor-1 inhibits autoantibody production via upregulation of apoptotic debris clearance by macrophages

Int J Rheum Dis. 2018 Dec;21(12):2071-2078. doi: 10.1111/1756-185X.13381. Epub 2018 Dec 16.


Aim: Allergy inhibitory receptor-1 (Allergin-1) is a newly identified immune regulatory molecule thought to influence autoantibody production. Autoantibody production, like that observed in Allergin-1-deficient mice, is crucial in the pathogenesis of several autoimmune diseases such as systemic lupus erythematosus. The purpose of this study is to clarify the regulatory role of Allergin-1-mediated autoantibody production using a murine model of thymocytic anaphylaxis.

Methods: C57BL/6 (WT) and Allergin-1-deficient mice were treated with apoptotic cells from naive thymocytes stimulated by dexamethasone. Antibody titers of total or immunoglobulin G (IgG) subclass of anti-double-stranded DNA (anti-dsDNA) and anti-histone antibody from serum were measured using an enzyme-linked immunosorbent assay. Macrophages from wild-type (WT) or Allergin-1-deficient mice were co-cultured with fluorescence-labeled apoptotic thymocytes or fluorogenic reagent and resultant phagocytic activity was quantified by with flow cytometry.

Results: After apoptotic cells injection, antibody titers of total and IgG3 anti-dsDNA and total anti-histone from serum were significantly increased in Allergin-1-deficient versus WT mice. Phagocytic activity was significantly lower in macrophages from Allergin-1-deficient mice versus WT mice.

Conclusion: Allergin-1 might play an inhibitory role in autoantibody production via upregulation of macrophage phagocytosis.

Keywords: Allergin-1; autoantibody; lupus nephritis; phagocytosis; systemic lupus erythematosus.

MeSH terms

  • Anaphylaxis / genetics
  • Anaphylaxis / immunology*
  • Anaphylaxis / metabolism
  • Anaphylaxis / pathology
  • Animals
  • Apoptosis*
  • Autoantibodies / blood
  • Autoantibodies / immunology*
  • Cells, Cultured
  • Coculture Techniques
  • Disease Models, Animal
  • Female
  • Macrophages / immunology*
  • Macrophages / metabolism
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Phagocytosis*
  • Receptors, Immunologic / deficiency
  • Receptors, Immunologic / genetics
  • Receptors, Immunologic / metabolism*
  • Thymocytes / immunology*
  • Thymocytes / pathology


  • Autoantibodies
  • Milr1 protein, mouse
  • Receptors, Immunologic