Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 10 (12)

Lactobacillus paracasei KW3110 Prevents Blue Light-Induced Inflammation and Degeneration in the Retina

Affiliations

Lactobacillus paracasei KW3110 Prevents Blue Light-Induced Inflammation and Degeneration in the Retina

Yuji Morita et al. Nutrients.

Abstract

Age-related macular degeneration and retinitis pigmentosa are leading causes of blindness and share a pathological feature, which is photoreceptor degeneration. To date, the lack of a potential treatment to prevent such diseases has raised great concern. Photoreceptor degeneration can be accelerated by excessive light exposure via an inflammatory response; therefore, anti-inflammatory agents would be candidates to prevent the progress of photoreceptor degeneration. We previously reported that a lactic acid bacterium, Lactobacillus paracasei KW3110 (L. paracasei KW3110), activated macrophages suppressing inflammation in mice and humans. Recently, we also showed that intake of L. paracasei KW3110 could mitigate visual display terminal (VDT) load-induced ocular disorders in humans. However, the biological mechanism of L. paracasei KW3110 to retain visual function remains unclear. In this study, we found that L. paracasei KW3110 activated M2 macrophages inducing anti-inflammatory cytokine interleukin-10 (IL-10) production in vitro using bone marrow-derived M2 macrophages. We also show that IL-10 gene expression was significantly increased in the intestinal immune tissues 6 h after oral administration of L. paracasei KW3110 in vivo. Furthermore, we demonstrated that intake of L. paracasei KW3110 suppressed inflammation and photoreceptor degeneration in a murine model of light-induced retinopathy. These results suggest that L. paracasei KW3110 may have a preventive effect against degrative retinal diseases.

Keywords: Lactobacillus paracasei KW3110; light; macrophage; retina.

Conflict of interest statement

Morita, Y., Fujiwara, D. and Kanauchi, O. are employed by Kirin Company Ltd. Jounai, K. is employed by Koiwai Dairy Products Co Ltd. The preventive effects of L. paracasei KW3110 have been applied for a patent (Application no. 2017-172620).

Figures

Figure 1
Figure 1
Effects of L. paracasei KW3110 on the activation of M2 macrophages and induction of IL-10 production. (A) Bone marrow-derived M2 macrophages were stimulated with lipo-teichoic acid (10 ng/mL), and L. paracasei KW3110 (0.1, 1, or 10 μg/mL) and the amounts of secreted IL-10 were measured by ELISA. (B) Relative IL-10 mRNA expression was measured using PCR. Values are represented as the mean ± SEM. Significance was assumed if the p value was < 0.05. ** p < 0.01. LTA, lipo-teichoic acid treated group; CTL, control; KW3110, L. paracasei KW3110 treated group.
Figure 2
Figure 2
Effect of L. paracasei KW3110 on the induction of M2 macrophage in the retina of a light-exposed mouse model. (A) Representative flow cytometry data of CD11b-positive and CD206-positive cells from the blue light-exposed mice fed a diet containing L. paracasei KW3110 (KW3110) or fed a control diet (CTL). (B) The ratio of CD11b-positive, f4/80-positive, and CD206-positive M2 macrophage cells to CD11b-positive and f4/80-positive macrophage cells. (C) The ratio of TNF-α-producing cells in CD11b-positive M1 macrophage cells. To detect inflammatory cytokine-producing cells, retinal cells were cultured under stimulation with Leukocyte Activation Cocktail plus BD GolgiPlug™ and analyzed by flow cytometry. (D) The production of inflammatory cytokines IL-1β and RANTES in the retinal cell culture medium. CTL, control; KW3110, L. paracasei KW3110; TNF-α, tumor necrosis factor-α; IL-1β, interleukin-1β; RANTES, regulated on activation, normal T cell expressed and secreted. Values are presented as the mean ± SEM. Significance was assumed if the p value was < 0.05. * p < 0.05.
Figure 3
Figure 3
A protective effect of L. paracasei KW3110 on light-induced histological retinal changes. (A) Hematoxylin and eosin staining of retinal sections. Arrow heads indicate the outer nuclear layer (ONL). Scale bar represents 100 µm. (B) ONL thickness was lower in mice fed a control diet than in mice fed a diet with L. paracasei KW3110. Values are presented as the mean ± SEM. Significance was assumed if the p value was < 0.05. *p < 0.05; **p < 0.01; non-light CTL, no light exposed mice group fed a control diet; light control, light exposed mice group fed a control diet; light KW3110, light exposed mice group fed a diet containing L. paracasei KW3110.
Figure 4
Figure 4
Suppression of visual function impairment by intake of L. paracasei KW3110. (A) The amplitude of a-waves in the scotopic ERG. (B) The amplitude of b-waves in the scotopic ERG. (C) The amplitude of b-waves in the photopic ERG. Results are represented as the mean ± SEM. * p < 0.05 for the control. CTL, control diet; KW3110, L. paracasei KW3110 diet.
Figure 4
Figure 4
Suppression of visual function impairment by intake of L. paracasei KW3110. (A) The amplitude of a-waves in the scotopic ERG. (B) The amplitude of b-waves in the scotopic ERG. (C) The amplitude of b-waves in the photopic ERG. Results are represented as the mean ± SEM. * p < 0.05 for the control. CTL, control diet; KW3110, L. paracasei KW3110 diet.

Similar articles

See all similar articles

Cited by 1 PubMed Central articles

  • Nutrition and Eye Health
    JG Lawrenson et al. Nutrients 11 (9). PMID 31489894.
    Diet is a key lifestyle factor that can have long-term effects on ocular health [...].

References

    1. Kishi S., Li D., Takahashi M., Hashimoto H. Photoreceptor damage after prolonged gazing at a computer game display. Jpn. J. Ophthalmol. 2010;54:514–516. doi: 10.1007/s10384-010-0849-2. - DOI - PubMed
    1. Suzuki M., Tsujikawa M., Itabe H., Du Z.J., Xie P., Matsumura N., Fu X., Zhang R., Sonoda K.H., Egashira K., et al. Chronic photo-oxidative stress and subsequent MCP-1 activation as causative factors for age-related macular degeneration. J. Cell Sci. 2012;125:2407–2415. doi: 10.1242/jcs.097683. - DOI - PMC - PubMed
    1. Glazer-Hockstein C., Dunaief J.L. Could blue light-blocking lenses decrease the risk of age-related macular degeneration? Retina. 2006;26:1–4. doi: 10.1097/00006982-200601000-00001. - DOI - PubMed
    1. Reme C.E., Grimm C., Hafezi F., Marti A., Wenzel A. Apoptotic cell death in retinal degenerations. Prog. Retin. Eye Res. 1998;17:443–464. doi: 10.1016/S1350-9462(98)00009-3. - DOI - PubMed
    1. Wong W.L., Su X., Li X., Cheung C.M., Klein R., Cheng C.Y., Wong T.Y. Global prevalence of age-related macular degeneration and disease burden projection for 2020 and 2040: A systematic review and meta-analysis. Lancet Glob. Health. 2014;2:106–116. doi: 10.1016/S2214-109X(13)70145-1. - DOI - PubMed

MeSH terms

Feedback