99mTc-Methyl-Diphosphonate Binding to Mineral Deposits in Cultures of Marrow-Derived Mesenchymal Stem Cells in Osteogenic Medium

Tissue Eng Part C Methods. 2019 Jan;25(1):49-57. doi: 10.1089/ten.TEC.2018.0299.


The work is notable for describing a highly sensitive, quantitative, and nondestructive method for evaluating the in vitro amount of mineral accompanying different types of osteogenic differentiation of mesenchymal stem cells in a monolayer cell culture. What is so unique and useful about the method is that it has the potential to be used to define the kinetics of the differentiation process, reflected in the mineralization, without destroying the monolayer. Therefore, it remains intact for further experiments.

Keywords: mesenchymal stem cells; mineral quantification; osteogenic differentiation; technetium.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Bone Marrow*
  • Calcification, Physiologic
  • Cell Differentiation*
  • Cells, Cultured
  • Goats
  • Humans
  • Mesenchymal Stem Cells / cytology*
  • Mesenchymal Stem Cells / metabolism
  • Minerals / metabolism*
  • Osteogenesis*
  • Radiopharmaceuticals / metabolism
  • Technetium Tc 99m Medronate / metabolism*


  • Minerals
  • Radiopharmaceuticals
  • Technetium Tc 99m Medronate