Abstract
A supramolecular host-guest FRET pair based on a carboxyfluorescein-labelled cucurbit[7]uril (CB7-CF, as acceptor) and the fluorescent dye 4',6-diamidino-2-phenylindole (DAPI, as donor) is developed for sensing of DNA. In comparison to the commercial DNA staining dye SYBR Green I, the new chemosensing ensemble offers dual-emission signals, which allows a linear ratiometric response over a wide concentration range.
MeSH terms
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Bridged-Ring Compounds / chemical synthesis
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Bridged-Ring Compounds / chemistry*
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DNA / analysis*
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DNA / chemistry
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Fluoresceins / chemical synthesis
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Fluoresceins / chemistry*
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Fluorescence
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Fluorescence Resonance Energy Transfer / methods
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Fluorescent Dyes / chemistry*
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Imidazoles / chemical synthesis
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Imidazoles / chemistry*
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Indoles / chemistry*
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Intercalating Agents / chemistry
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Limit of Detection
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Spectrometry, Fluorescence
Substances
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Bridged-Ring Compounds
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Fluoresceins
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Fluorescent Dyes
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Imidazoles
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Indoles
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Intercalating Agents
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cucurbit(7)uril
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DAPI
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4-carboxyfluorescein
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DNA