Four new species of Enlinia Aldrich are described from Chile: Enlinia biobio n. sp., Enlinia chilensis n. sp., Enlinia enormis n. sp., and Enlinia isoloba n. sp. These specimens were collected during a 2013 invertebrate survey in sclerophyll and Valdivian temperate rain forest habitats of the central and southern Chilean Andes. The only other species of Enlinia recorded from Chile is E. atrata (Van Duzee). Photos of holotypes and type localities and a key to the five species known to occur in Chile are provided.
Keywords: Andes; Chile; Enlinia; Enliniinae; Neotropical; South America; Valdivian temperate rain forest; micro-dolichopodids; new species.