A novel two colour ELISPOT assay. I. Simultaneous detection of distinct types of antibody-secreting cells

J Immunol Methods. 1988 Nov 25;115(1):31-7. doi: 10.1016/0022-1759(88)90306-7.


A novel assay system has been developed which is based on the ELISPOT methodology and employs a combination of two immunoenzyme visualization systems yielding distinct colour products. This variation permits the simultaneous enumeration of two different types of cell secreting antigenically distinct products. Optimal conditions for the concurrent detection of human mononuclear cells secreting IgG or IgA antibodies are described.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Antibodies, Viral / biosynthesis
  • Antibody-Producing Cells / analysis*
  • Chromogenic Compounds*
  • Enzyme-Linked Immunosorbent Assay*
  • Hemolytic Plaque Technique*
  • Humans
  • Immunoglobulin A / biosynthesis
  • Immunoglobulin G / biosynthesis
  • Indicators and Reagents
  • Influenza A virus / immunology


  • Antibodies, Viral
  • Chromogenic Compounds
  • Immunoglobulin A
  • Immunoglobulin G
  • Indicators and Reagents