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. 2019 Jun;139(6):1404-1407.
doi: 10.1016/j.jid.2018.12.002. Epub 2018 Dec 17.

A Western Diet, but Not a High-Fat and Low-Sugar Diet, Predisposes Mice to Enhanced Susceptibility to Imiquimod-Induced Psoriasiform Dermatitis

Sebastian Yu  1 , Xuesong Wu  2 , Yan Zhou  2 , Lili Sheng  3 , Prasant Kumar Jena  3 , Dan Han  2 , Yu Jui Yvonne Wan  3 , Samuel T Hwang  4
Affiliations

A Western Diet, but Not a High-Fat and Low-Sugar Diet, Predisposes Mice to Enhanced Susceptibility to Imiquimod-Induced Psoriasiform Dermatitis

Sebastian Yu et al. J Invest Dermatol. 2019 Jun.
No abstract available

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Conflict of interest statement

CONFLICT OF INTEREST

The authors declare no conflict of interest.

Figures

Figure 1.
Figure 1.. WD-fed mice have enhanced susceptibility to psoriasiform dermatitis and higher expression levels of neutrophil markers and developed more Munro microabscesses in response to IMQ treatment.
(a) Mice fed with high fat diet gained more body weight than those fed with Western diet, low fat diet, or control diet. (b) Photographs of mice in four different diet groups after feeding for 16 weeks. (c) At week 12, mice fed with WD had significantly higher ear thickness change than mice fed with HFD on day 4 and day 5 of the IMQ course. No difference in ear thickness change was observed between HFD mice and LFD mice on day 5. (d) At week 16, WD-fed mice had more prominent ear thickness change than HFD-fed mice from day 3 to day 5 of the IMQ course. There was no difference in ear thickness change between HFD and LFD mice on day 5. (e) Epidermal thickness after 5-day IMQ or vehicle treatment as measured in microscopic fields. Vehicles-treated ears of HFD and WD mice were thicker than LFD and CD mice, respectively. IMQ-treated ears of WD mice were thicker than those of HFD mice. Vehicles-treated ears of WD mice were also thicker than those of HFD mice, although the difference did not reach statistical significance. (f) Representative photos of H&E staining of ears after 5-day IMQ treatment in four groups (bar, 50 μm). More cell infiltrates and parakeratosis were observed in WD mice. A wild-type (WT) mouse ear treated with 5-day vehicle cream was shown for comparison. (g, h) While there was no difference in Cxcl1 expression between WD-fed and HFD-fed mice, WD-fed mice had higher expression of Cxcl2 after 5-day IMQ treatment. (i) WD-fed mice had the highest mRNA expression of the neutrophil marker Ly6g. (j) Consistent with the highest expression of Ly6g, there were more Munro microabscesses observed in WD-fed mice. (k) More Gr-1 positive cells were observed in WD-IMQ group than other three IMQ groups. (l) Representative photos of Gr-1 immunohistochemistry. Black scale bar: 20 μm. HFD, high fat diet. LFD, low fat diet. WD, Western diet. CD, control diet. IMQ, imiquimod. * P<0.05. ** P<0.01. *** P<0.001. **** P<0.0001
Figure 2.
Figure 2.. WD-fed mice have the highest baseline mRNA expression of Il-17a and, after IMQ 5-day course, have the highest mRNA expressions levels of Nlrp3 and Il-1b among the four dietary groups.
(a) IMQ treatment induced higher expression levels of Il-17a in all four dietary groups. Then we examined baseline expression of Il-17a in vehicle-treated ears. WD induced a 45-fold increase of Il-17a compared with CD while HFD induced only 20-fold increase (WD vs. HFD, P=0.050). (b, c) WD and HFD did not increase expression levels of Il-23 and Il-22 in both IMQ- and vehicle-treated ears. (d, e) After IMQ 5-day course, WD-fed mice had higher expression of Nlrp3 and Il-1b than HFD-fed mice. (f) There was no difference in the expression of Tnf-a among four dietary groups. IMQ, imiquimod. NS, no statistical significance. * P<0.05. ** P<0.01.
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